%0 Journal Article %1 He.2002 %A He, Y. %A Kozaki, K. %A Karpanen, T. %A Koshikawa, K. %A Yla-Herttuala, S. %A Takahashi, T. %A Alitalo, K. %D 2002 %J J.Natl.Cancer Inst. %K & Animals Antibodies Blotting C Cell Cells Cultured D Division Endothelial Expression Factor Factors Female Gene Growth Human Humans Kinase Kinases Laboratories Line Lung Lymph Lymphangiogenesis Lymphatic Metastasis Mice Neoplasm Neoplasms Neoplastic Nodes Protein-Tyrosine RNA Receptor Receptor-3 Receptors Regulation Research Scid Signal Time Transduction Transfection Transplantation Tumor Vascular Vessels Western antagonists blood cells control genetics immunology inhibitors metabolism methods pathology prevention protein secondary supply %N 11 %P 819-825 %T Suppression of tumor lymphangiogenesis and lymph node metastasis by blocking vascular endothelial growth factor receptor 3 signaling %U PM:12048269 %V 94 %X BACKGROUND: Vascular endothelial growth factor C (VEGF-C) stimulates tumor lymphangiogenesis (i.e., formation of lymphatic vessels) and metastasis to regional lymph nodes by interacting with VEGF receptor 3 (VEGFR-3). We sought to determine whether inhibiting VEGFR-3 signaling, and thus tumor lymphangiogenesis, would inhibit tumor metastasis. METHODS: We used the highly metastatic human lung cancer cell line NCI-H460-LNM35 (LNM35) and its parental line NCI-H460-N15 (N15) with low metastatic capacity. We inserted genes by transfection and established a stable N15 cell line secreting VEGF-C and a LNM35 cell line secreting the soluble fusion protein VEGF receptor 3-immunoglobulin (VEGFR-3-Ig, which binds VEGF-C and inhibits VEGFR-3 signaling). Control lines were transfected with mock vectors. Tumor cells were implanted subcutaneously into severe combined immunodeficient mice (n = 6 in each group), and tumors and metastases were examined 6 weeks later. In another approach, recombinant aden

@article{He.2002, abstract = {BACKGROUND: Vascular endothelial growth factor C (VEGF-C) stimulates tumor lymphangiogenesis (i.e., formation of lymphatic vessels) and metastasis to regional lymph nodes by interacting with VEGF receptor 3 (VEGFR-3). We sought to determine whether inhibiting VEGFR-3 signaling, and thus tumor lymphangiogenesis, would inhibit tumor metastasis. METHODS: We used the highly metastatic human lung cancer cell line NCI-H460-LNM35 (LNM35) and its parental line NCI-H460-N15 (N15) with low metastatic capacity. We inserted genes by transfection and established a stable N15 cell line secreting VEGF-C and a LNM35 cell line secreting the soluble fusion protein VEGF receptor 3-immunoglobulin (VEGFR-3-Ig, which binds VEGF-C and inhibits VEGFR-3 signaling). Control lines were transfected with mock vectors. Tumor cells were implanted subcutaneously into severe combined immunodeficient mice (n = 6 in each group), and tumors and metastases were examined 6 weeks later. In another approach, recombinant aden}, added-at = {2010-02-05T11:28:39.000+0100}, author = {He, Y. and Kozaki, K. and Karpanen, T. and Koshikawa, K. and Yla-Herttuala, S. and Takahashi, T. and Alitalo, K.}, biburl = {https://www.bibsonomy.org/bibtex/2058093fbd306ed90ab831b5958d7b0aa/kanefendt}, interhash = {68e46439e1fbf3260cc500b417c929ef}, intrahash = {058093fbd306ed90ab831b5958d7b0aa}, journal = {J.Natl.Cancer Inst.}, keywords = {& Animals Antibodies Blotting C Cell Cells Cultured D Division Endothelial Expression Factor Factors Female Gene Growth Human Humans Kinase Kinases Laboratories Line Lung Lymph Lymphangiogenesis Lymphatic Metastasis Mice Neoplasm Neoplasms Neoplastic Nodes Protein-Tyrosine RNA Receptor Receptor-3 Receptors Regulation Research Scid Signal Time Transduction Transfection Transplantation Tumor Vascular Vessels Western antagonists blood cells control genetics immunology inhibitors metabolism methods pathology prevention protein secondary supply}, number = 11, pages = {819-825}, timestamp = {2010-02-05T11:28:39.000+0100}, title = {Suppression of tumor lymphangiogenesis and lymph node metastasis by blocking vascular endothelial growth factor receptor 3 signaling}, url = {PM:12048269}, volume = 94, year = 2002 }