%0 Journal Article %1 kmpfer_nocardia_2007 %A Kämpfer, Peter %A Huber, Birgit %A Buczolits, Sandra %A Thummes, Kathrin %A Grün-Wollny, Iris %A Busse, Hans-Jürgen %D 2007 %J International journal of systematic and evolutionary microbiology %K 16S Bacterial Bacterial_Typing_Techniques DNA Genes IFZ Italy Lipids Molecular_Sequence_Data Nocardia Nucleic_Acid Nucleic_Acid_Hybridization Phylogeny Quinones RNA Ribosomal Sequence_Analysis Sequence_Homology Soil_Microbiology Spores rRNA %P 1183-7 %T Nocardia acidivorans sp. nov., isolated from soil of the island of Stromboli %V 57 %X A Gram-positive, non-spore-forming bacterium (strain GW4-1778(T)) was isolated from soil of the Italian island of Stromboli. 16S rRNA gene sequence similarity studies showed that strain GW4-1778(T) is a member of the genus Nocardia, most closely related to Nocardia pseudobrasiliensis (GenBank accession no. DQ659914; 98.6 \%), Nocardia nova (Z36930; 98.6 \%), Nocardia niigatensis (AB092563; 98.4 \%), Nocardia jiangxiensis (AY639902; 98.0 \%), Nocardia uniformis (Z46752; 98.0 \%) and Nocardia miyunensis (AY639901; 97.8 \%). Strain GW4-1778(T) could be distinguished from any other established Nocardia species by sequence similarity values of less than 97.5 \%. Strain GW4-1778(T) exhibited a quinone system with the predominant compound MK-8 (H(4), omega-cycl) (99.5 \%) and traces of MK-8 (H(4)), characteristic for the genus Nocardia. The polar lipid profile of strain GW4-1778(T) consisted of the predominant compound diphosphatidylglycerol, moderate amounts of phosphatidylethanolamine, phosphatidylinositol, two phosphatidylinositol mannosides, a unknown polar lipid and trace amounts of two unknown lipids and the major fatty acids were C(15 : 0), C(16 : 0), C(17 : 1)omega8c and 10-methyl C(17 : 0). The results of DNA-DNA hybridizations and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain GW4-1778(T) from related species with 16S rRNA gene similarities of \textgreater97.5 \%. Therefore, strain GW4-1778(T) merits species status, for which the name Nocardia acidivorans sp. nov. is proposed, with the type strain GW4-1778(T) (=CCUG 53410(T)=CIP 109315(T)=DSM 45049(T)).

@article{kmpfer_nocardia_2007, abstract = {A Gram-positive, non-spore-forming bacterium (strain GW4-1778(T)) was isolated from soil of the Italian island of Stromboli. 16S rRNA gene sequence similarity studies showed that strain GW4-1778(T) is a member of the genus Nocardia, most closely related to Nocardia pseudobrasiliensis (GenBank accession no. DQ659914; 98.6 \%), Nocardia nova (Z36930; 98.6 \%), Nocardia niigatensis (AB092563; 98.4 \%), Nocardia jiangxiensis (AY639902; 98.0 \%), Nocardia uniformis (Z46752; 98.0 \%) and Nocardia miyunensis (AY639901; 97.8 \%). Strain GW4-1778(T) could be distinguished from any other established Nocardia species by sequence similarity values of less than 97.5 \%. Strain GW4-1778(T) exhibited a quinone system with the predominant compound MK-8 (H(4), omega-cycl) (99.5 \%) and traces of MK-8 (H(4)), characteristic for the genus Nocardia. The polar lipid profile of strain GW4-1778(T) consisted of the predominant compound diphosphatidylglycerol, moderate amounts of phosphatidylethanolamine, phosphatidylinositol, two phosphatidylinositol mannosides, a unknown polar lipid and trace amounts of two unknown lipids and the major fatty acids were C(15 : 0), C(16 : 0), C(17 : 1)omega8c and 10-methyl C(17 : 0). The results of DNA-DNA hybridizations and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain GW4-1778(T) from related species with 16S rRNA gene similarities of {\textgreater}97.5 \%. Therefore, strain GW4-1778(T) merits species status, for which the name Nocardia acidivorans sp. nov. is proposed, with the type strain GW4-1778(T) (=CCUG 53410(T)=CIP 109315(T)=DSM 45049(T)).}, added-at = {2008-05-07T16:02:06.000+0200}, author = {Kämpfer, Peter and Huber, Birgit and Buczolits, Sandra and Thummes, Kathrin and Grün-Wollny, Iris and Busse, Hans-Jürgen}, biburl = {https://www.bibsonomy.org/bibtex/26d01f467b51fa2f6506e820ba7b9eccd/recymikrobio}, interhash = {7c533792118a86d9d9268f899dced58b}, intrahash = {6d01f467b51fa2f6506e820ba7b9eccd}, issn = {14665026}, journal = {International journal of systematic and evolutionary microbiology}, keywords = {16S Bacterial Bacterial_Typing_Techniques DNA Genes IFZ Italy Lipids Molecular_Sequence_Data Nocardia Nucleic_Acid Nucleic_Acid_Hybridization Phylogeny Quinones RNA Ribosomal Sequence_Analysis Sequence_Homology Soil_Microbiology Spores rRNA}, month = {June}, note = {PMID: 17551026}, pages = {1183-7}, timestamp = {2008-07-18T13:58:07.000+0200}, title = {Nocardia acidivorans sp. nov., isolated from soil of the island of Stromboli}, volume = 57, year = 2007 }