Abstract
The rapid development of molecular genetic analysis tools has made it possible
to analyze most biological material even they are in a small quantity. Many different
extraction protocols are being used to isolate DNA. However not all extraction methods
perform equally well with heavily inhibited factors and sometimes is very important to
choose an extraction procedure that could extract DNA efficiency and had the ability to
minimize the amount of inhibitors co-extracted with the sample.
Two different extraction methods were chosen: Chelex and Organic Extraction.
To evaluate the efficiency of the procedures, quantity of DNA is compared using TaqMan
Probe & Absolute Quantification method in ABI Prism RT-PCR. The samples used were
blood samples.
The results showed that with both methods extraction was efficiency for further
analysis, but concentration of DNA was greater extracted with Chelex than it with
Organic Extraction.
Compare with Organic Extraction, Chelex is more efficient, more rapid,
inexpensive, involves fewer steps and thus fewer opportunities for sample to sample
contamination, no hazard chemicals are used.
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