%0 Journal Article %1 olsen_comparison_1999 %A Olsen, C C %A Schwebke, J R %A Benjamin, W H %A Beverly, A %A Waites, K B %D 1999 %J Journal of Clinical Microbiology %K Adult, Agar, Bacteriological Charcoal, Culture Evaluation False Female, Gonorrhea, Humans, Media, Negative Neisseria Reactions, Sensitivity Smears Specificity, Studies Techniques, Topic, Vaginal and as gonorrhoeae, %N 11 %P 3583--3585 %T Comparison of direct inoculation and Copan transport systems for isolation of Neisseria gonorrhoeae from endocervical specimens %U http://www.ncbi.nlm.nih.gov/pubmed/10523556 %V 37 %X Two commercial swab transport systems, Copan Amies gel agar with and without charcoal (Copan Diagnostics, Corona, Calif.), were compared to direct inoculation onto modified Thayer-Martin medium for detection of Neisseria gonorrhoeae in 1,490 endocervical specimens obtained from women attending a sexually transmitted disease clinic. Copan swabs were held in the transport system for 24 h at room temperature prior to inoculation onto modified Thayer-Martin medium. All cultures were incubated at 35 degrees C in 5\% CO(2), and bacteria were identified on the basis of Gram stain, oxidase, and biochemical reactions. Copan Amies gel agar transport system without charcoal detected 77 of 81 (95\%) direct inoculation culture-positive specimens, and Copan Amies gel agar transport system with charcoal detected 53 of 56 (95\%) directly inoculated culture-positive specimens. Copan Amies gel agar without charcoal inoculated after 6 h supported growth of 56 (98\%) positive cultures out of only 55 directly inoculated culture-positive specimens. This study demonstrates that Copan swabs represent a reasonable alternative, providing convenience, low cost, and ease of use while still maintaining a satisfactory recovery rate of N. gonorrhoeae from clinical specimens, if specimens can be inoculated onto selective media within a relatively short time period not involving overnight shipment.

@article{olsen_comparison_1999, abstract = {Two commercial swab transport systems, Copan Amies gel agar with and without charcoal {(Copan} Diagnostics, Corona, Calif.), were compared to direct inoculation onto modified {Thayer-Martin} medium for detection of Neisseria gonorrhoeae in 1,490 endocervical specimens obtained from women attending a sexually transmitted disease clinic. Copan swabs were held in the transport system for 24 h at room temperature prior to inoculation onto modified {Thayer-Martin} medium. All cultures were incubated at 35 degrees C in 5\% {CO(2),} and bacteria were identified on the basis of Gram stain, oxidase, and biochemical reactions. Copan Amies gel agar transport system without charcoal detected 77 of 81 (95\%) direct inoculation culture-positive specimens, and Copan Amies gel agar transport system with charcoal detected 53 of 56 (95\%) directly inoculated culture-positive specimens. Copan Amies gel agar without charcoal inoculated after 6 h supported growth of 56 (98\%) positive cultures out of only 55 directly inoculated culture-positive specimens. This study demonstrates that Copan swabs represent a reasonable alternative, providing convenience, low cost, and ease of use while still maintaining a satisfactory recovery rate of N. gonorrhoeae from clinical specimens, if specimens can be inoculated onto selective media within a relatively short time period not involving overnight shipment.}, added-at = {2011-03-11T10:05:34.000+0100}, author = {Olsen, C C and Schwebke, J R and Benjamin, W H and Beverly, A and Waites, K B}, biburl = {https://www.bibsonomy.org/bibtex/2f29c9b8dc9b8ed2a61573d2e33c86e83/jelias}, interhash = {d75f4065b60d538d7dbfa132948fbf90}, intrahash = {f29c9b8dc9b8ed2a61573d2e33c86e83}, issn = {0095-1137}, journal = {Journal of Clinical Microbiology}, keywords = {Adult, Agar, Bacteriological Charcoal, Culture Evaluation False Female, Gonorrhea, Humans, Media, Negative Neisseria Reactions, Sensitivity Smears Specificity, Studies Techniques, Topic, Vaginal and as gonorrhoeae,}, month = nov, note = {{PMID:} 10523556}, number = 11, pages = {3583--3585}, timestamp = {2011-03-11T10:05:43.000+0100}, title = {Comparison of direct inoculation and Copan transport systems for isolation of Neisseria gonorrhoeae from endocervical specimens}, url = {http://www.ncbi.nlm.nih.gov/pubmed/10523556}, volume = 37, year = 1999 }