Biological macromolecules evolve and function within intracellular
environments that are crowded with other macromolecules. Crowding
results in surprisingly large quantitative effects on both the rates
and the equilibria of interactions involving macromolecules, but
such interactions are commonly studied outside the cell in uncrowded
buffers. The addition of high concentrations of natural and synthetic
macromolecules to such buffers enables crowding to be mimicked in
vitro, and should be encouraged as a routine variable to study. The
stimulation of protein aggregation by crowding might account for
the existence of molecular chaperones that combat this effect. Positive
results of crowding include enhancing the collapse of polypeptide
chains into functional proteins, the assembly of oligomeric structures
and the efficiency of action of some molecular chaperones and metabolic
pathways.
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%0 Journal Article
%1 Elli_2001_597
%A Ellis, R. J.
%D 2001
%J Trends Biochem. Sci.
%K 11590012 80 Accelerators, Adenocarcinoma, Aged, Analysis, Animals, Assurance, Biological, Carboxylase, Care, Chaperones, Combined Conformal, Development, Escherichia Factors, Folding, Health Humans, In Indium Intraoperative Kinetics, Macromolecular Male, Middle Mobile Modality Models, Molecular Neoplasms, Nursing, Ohio, Operating Outcome, Palladium, Particle Period, Perioperative Preoperative Program Prostatic Protein Proteins, Quality Radioimmunodetection, Radioisotopes, Radiotherapy, Ribulose-Bisphosphate Risk Rooms, Substances, Sulfurtransferase, Survival Therapy, Thermodynamics, Thiosulfate Treatment Units, Vitro, and coli, over,
%N 10
%P 597--604
%T Macromolecular crowding: obvious but underappreciated.
%U http://dx.doi.org/10.1016/S0968-0004(01)01938-7
%V 26
%X Biological macromolecules evolve and function within intracellular
environments that are crowded with other macromolecules. Crowding
results in surprisingly large quantitative effects on both the rates
and the equilibria of interactions involving macromolecules, but
such interactions are commonly studied outside the cell in uncrowded
buffers. The addition of high concentrations of natural and synthetic
macromolecules to such buffers enables crowding to be mimicked in
vitro, and should be encouraged as a routine variable to study. The
stimulation of protein aggregation by crowding might account for
the existence of molecular chaperones that combat this effect. Positive
results of crowding include enhancing the collapse of polypeptide
chains into functional proteins, the assembly of oligomeric structures
and the efficiency of action of some molecular chaperones and metabolic
pathways.
@article{Elli_2001_597,
abstract = {Biological macromolecules evolve and function within intracellular
environments that are crowded with other macromolecules. Crowding
results in surprisingly large quantitative effects on both the rates
and the equilibria of interactions involving macromolecules, but
such interactions are commonly studied outside the cell in uncrowded
buffers. The addition of high concentrations of natural and synthetic
macromolecules to such buffers enables crowding to be mimicked in
vitro, and should be encouraged as a routine variable to study. The
stimulation of protein aggregation by crowding might account for
the existence of molecular chaperones that combat this effect. Positive
results of crowding include enhancing the collapse of polypeptide
chains into functional proteins, the assembly of oligomeric structures
and the efficiency of action of some molecular chaperones and metabolic
pathways.},
added-at = {2009-06-03T11:20:58.000+0200},
author = {Ellis, R. J.},
biburl = {https://www.bibsonomy.org/bibtex/24ed2cf06f0dca0bc4468508ea4cc9b92/hake},
description = {The whole bibliography file I use.},
file = {Elli_2001_597.pdf:Elli_2001_597.pdf:PDF},
interhash = {1501b465a4e71f69242983559e865e39},
intrahash = {4ed2cf06f0dca0bc4468508ea4cc9b92},
journal = {Trends Biochem. Sci.},
key = 175,
keywords = {11590012 80 Accelerators, Adenocarcinoma, Aged, Analysis, Animals, Assurance, Biological, Carboxylase, Care, Chaperones, Combined Conformal, Development, Escherichia Factors, Folding, Health Humans, In Indium Intraoperative Kinetics, Macromolecular Male, Middle Mobile Modality Models, Molecular Neoplasms, Nursing, Ohio, Operating Outcome, Palladium, Particle Period, Perioperative Preoperative Program Prostatic Protein Proteins, Quality Radioimmunodetection, Radioisotopes, Radiotherapy, Ribulose-Bisphosphate Risk Rooms, Substances, Sulfurtransferase, Survival Therapy, Thermodynamics, Thiosulfate Treatment Units, Vitro, and coli, over,},
month = Oct,
number = 10,
pages = {597--604},
pii = {S0968000401019387},
pmid = {11590012},
timestamp = {2009-06-03T11:21:11.000+0200},
title = {Macromolecular crowding: obvious but underappreciated.},
url = {http://dx.doi.org/10.1016/S0968-0004(01)01938-7},
volume = 26,
year = 2001
}