A new PCR protocol for molecular typing of Haemophilus influenzae serotype e (Hie) was developed. To this end, the sequence of the cap region II of Hie strain ATCC8142 was identified, which was \textgreater99\% identical to the recently published sequence of Hie isolate 274. The PCR using primer pair TTL63/TTL64 amplifies an internal 592-bp fragment of ecs4, an e-specific capsule synthesis gene, in 40 of 40 Hie strains. Of all non-Hie strains, there were no false positives. False-negative results of the PCR proposed by Falla et al. (1994) are explained by single nucleotide insertions in the primer sequences.
%0 Journal Article
%1 lam_new_2011
%A Lâm, Thiên-Trí
%A Elias, Johannes
%A Frosch, Matthias
%A Vogel, Ulrich
%A Claus, Heike
%D 2011
%J International Journal of Medical Microbiology: IJMM
%K ag_vogel imported
%N 2
%P 176--179
%R 10.1016/j.ijmm.2010.07.004
%T New diagnostic PCR for Haemophilus influenzae serotype e based on the cap locus of strain ATCC 8142
%U http://www.ncbi.nlm.nih.gov/pubmed/20940106
%V 301
%X A new PCR protocol for molecular typing of Haemophilus influenzae serotype e (Hie) was developed. To this end, the sequence of the cap region II of Hie strain ATCC8142 was identified, which was \textgreater99\% identical to the recently published sequence of Hie isolate 274. The PCR using primer pair TTL63/TTL64 amplifies an internal 592-bp fragment of ecs4, an e-specific capsule synthesis gene, in 40 of 40 Hie strains. Of all non-Hie strains, there were no false positives. False-negative results of the PCR proposed by Falla et al. (1994) are explained by single nucleotide insertions in the primer sequences.
@article{lam_new_2011,
abstract = {A new {PCR} protocol for molecular typing of Haemophilus influenzae serotype e {(Hie)} was developed. To this end, the sequence of the cap region {II} of Hie strain {ATCC8142} was identified, which was {\textgreater}99\% identical to the recently published sequence of Hie isolate 274. The {PCR} using primer pair {TTL63/TTL64} amplifies an internal 592-bp fragment of ecs4, an e-specific capsule synthesis gene, in 40 of 40 Hie strains. Of all {non-Hie} strains, there were no false positives. False-negative results of the {PCR} proposed by Falla et al. (1994) are explained by single nucleotide insertions in the primer sequences.},
added-at = {2011-04-07T15:44:20.000+0200},
author = {Lâm, {Thiên-Trí} and Elias, Johannes and Frosch, Matthias and Vogel, Ulrich and Claus, Heike},
biburl = {https://www.bibsonomy.org/bibtex/20f8240b68762942ac75dc9cd4f62f500/hymi},
doi = {10.1016/j.ijmm.2010.07.004},
interhash = {e098689a78df8c4a5cecbc92a6606f06},
intrahash = {0f8240b68762942ac75dc9cd4f62f500},
issn = {1618-0607},
journal = {International Journal of Medical Microbiology: {IJMM}},
keywords = {ag_vogel imported},
month = feb,
note = {{PMID:} 20940106},
number = 2,
pages = {176--179},
timestamp = {2011-04-07T15:47:41.000+0200},
title = {New diagnostic {PCR} for Haemophilus influenzae serotype e based on the cap locus of strain {ATCC} 8142},
url = {http://www.ncbi.nlm.nih.gov/pubmed/20940106},
volume = 301,
year = 2011
}