The Arabidopsis double pore K+ channel KCO1 was fused to green fluorescent protein and expressed in tobacco protoplasts. Microscopic analysis revealed a bright green fluorescence at the vacuolar membrane. RT-PCR experiments showed that KCO1 is expressed in the mesophyll. Vacuoles; from Arabidopsis wild-type and kco1 knockout plants were isolated for patch-clamp analyses. Currents mediated by slow-activating vacuolar (SV) channels of mesophyll cell vacuoles were significantly smaller in kco1 plants compared to the wild-type. This shown that KCO1 is involved in the formation of SV channels. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
%0 Journal Article
%1 RN1191
%A Schönknecht, G.
%A Spoormaker, P.
%A Steinmeyer, R.
%A Brüggeman, L.
%A Ache, P.
%A Dutta, R.
%A Reintanz, B.
%A Godde, M.
%A Hedrich, R.
%A Palme, K.
%D 2002
%J Febs Letters
%K channel domain k+ myOwn two-pore
%N 1-3
%P 28-32
%R Doi 10.1016/S0014-5793(01)03273-2
%T KCO1 is a component of the slow-vacuolar (SV) ion channel
%U /brokenurl#<Go to ISI>://WOS:000173628000006
%V 511
%X The Arabidopsis double pore K+ channel KCO1 was fused to green fluorescent protein and expressed in tobacco protoplasts. Microscopic analysis revealed a bright green fluorescence at the vacuolar membrane. RT-PCR experiments showed that KCO1 is expressed in the mesophyll. Vacuoles; from Arabidopsis wild-type and kco1 knockout plants were isolated for patch-clamp analyses. Currents mediated by slow-activating vacuolar (SV) channels of mesophyll cell vacuoles were significantly smaller in kco1 plants compared to the wild-type. This shown that KCO1 is involved in the formation of SV channels. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
@article{RN1191,
abstract = {The Arabidopsis double pore K+ channel KCO1 was fused to green fluorescent protein and expressed in tobacco protoplasts. Microscopic analysis revealed a bright green fluorescence at the vacuolar membrane. RT-PCR experiments showed that KCO1 is expressed in the mesophyll. Vacuoles; from Arabidopsis wild-type and kco1 knockout plants were isolated for patch-clamp analyses. Currents mediated by slow-activating vacuolar (SV) channels of mesophyll cell vacuoles were significantly smaller in kco1 plants compared to the wild-type. This shown that KCO1 is involved in the formation of SV channels. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.},
added-at = {2024-02-14T14:38:32.000+0100},
author = {Schönknecht, G. and Spoormaker, P. and Steinmeyer, R. and Brüggeman, L. and Ache, P. and Dutta, R. and Reintanz, B. and Godde, M. and Hedrich, R. and Palme, K.},
biburl = {https://www.bibsonomy.org/bibtex/2396192dbb4cedf5ac9642377a81a16e7/rainerhedrich_2},
doi = {Doi 10.1016/S0014-5793(01)03273-2},
interhash = {c16fb54b94f59afe87f3e0c2e3914e5d},
intrahash = {396192dbb4cedf5ac9642377a81a16e7},
issn = {0014-5793},
journal = {Febs Letters},
keywords = {channel domain k+ myOwn two-pore},
note = {517uf
Times Cited:49
Cited References Count:27},
number = {1-3},
pages = {28-32},
timestamp = {2024-02-14T14:38:32.000+0100},
title = {KCO1 is a component of the slow-vacuolar (SV) ion channel},
type = {Journal Article},
url = {/brokenurl#<Go to ISI>://WOS:000173628000006},
volume = 511,
year = 2002
}