Dopamine transmission is involved in reward processing and motor control, and its impairment plays a central role in numerous neurological disorders. Despite its strong pathophysiological relevance, the molecular and structural organization of the dopaminergic synapse remains to be established. Here, we used targeted labelling and fluorescence activated sorting to purify striatal dopaminergic synaptosomes. We provide the proteome of dopaminergic synapses with 57 proteins specifically enriched. Beyond canonical markers of dopamine neurotransmission such as dopamine biosynthetic enzymes and cognate receptors, we validated 6 proteins not previously described as enriched. Moreover, our data reveal the adhesion of dopaminergic synapses to glutamatergic, GABAergic or cholinergic synapses in structures we named “dopamine hub synapses”. At glutamatergic synapses, pre- and postsynaptic markers are significantly increased upon association with dopamine synapses. Dopamine hub synapses may thus support local dopaminergic signalling, complementing volume transmission thought to be the major mechanism by which monoamines modulate network activity. The neurotransmitter dopamine is an important regulator of brain function. Here the authors describe “dopamine hub synapses”, where dopamine transmission may act in synergy with other neurotransmitters.
"So, for example, chocolate increases dopamine above baseline about 50%. Sex is about a 100%. Nicotine is about 150%. And amphetamines is about 1,000%."
Collectif. Santé mentale, (January 2013)Dossier réalisé en partenariat avec Clipsya (Centre Psycho-Alimentaire). Il reprend pour partie des interventions de la 1ère journée de formation scientifique :Öbésités : un autre regard sur un nouveau fléau"..
J. Raymond, A. Fargin, M. Lohse, J. Regan, S. Senogles, R. Lefkowitz, and M. Caron. Mol Pharmacol, 36 (1):
15-21(July 1989)Raymond, J R Fargin, A Lohse, M J Regan, J W Senogles, S E Lefkowitz,
R J Caron, M G HL-16037/HL/NHLBI NIH HHS/United States NS-19576/NS/NINDS
NIH HHS/United States S07-RR-05405/RR/NCRR NIH HHS/United States
Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.
United states Molecular pharmacology Mol Pharmacol. 1989 Jul;36(1):15-21..