We have developed a rapid and simple method for isolating human peripheral blood monocytes in suspension. The procedure combines two separation media and involves isolation of the mononuclear cells by centrifugation through Ficoll-Hypaque followed by purification of the monocytes using Sepracell-MN, a colloidal silica-based medium. The final cell population contained approximately 90% monocytes with good functional ability. The contaminating cells were lymphocytes. Viability was always greater than or equal to 99% with 90% recovery of the monocytes from the mononuclear cells
%0 Journal Article
%1 Vissers.1988
%A Vissers, M. C.
%A Jester, S. A.
%A Fantone, J. C.
%D 1988
%J J.Immunol.Methods
%K Cell Centrifugation Colloids Count Density Diatrizoate Dioxide Ficoll Gradient Human Humans Leukocyte Lymphocytes Monocytes Polysaccharides Population Separation Silicon blood cells methods pathology
%N 2
%P 203-207
%T Rapid purification of human peripheral blood monocytes by centrifugation through Ficoll-Hypaque and Sepracell-MN
%U PM:2837516
%V 110
%X We have developed a rapid and simple method for isolating human peripheral blood monocytes in suspension. The procedure combines two separation media and involves isolation of the mononuclear cells by centrifugation through Ficoll-Hypaque followed by purification of the monocytes using Sepracell-MN, a colloidal silica-based medium. The final cell population contained approximately 90% monocytes with good functional ability. The contaminating cells were lymphocytes. Viability was always greater than or equal to 99% with 90% recovery of the monocytes from the mononuclear cells
@article{Vissers.1988,
abstract = {We have developed a rapid and simple method for isolating human peripheral blood monocytes in suspension. The procedure combines two separation media and involves isolation of the mononuclear cells by centrifugation through Ficoll-Hypaque followed by purification of the monocytes using Sepracell-MN, a colloidal silica-based medium. The final cell population contained approximately 90% monocytes with good functional ability. The contaminating cells were lymphocytes. Viability was always greater than or equal to 99% with 90% recovery of the monocytes from the mononuclear cells},
added-at = {2010-02-05T11:28:39.000+0100},
author = {Vissers, M. C. and Jester, S. A. and Fantone, J. C.},
biburl = {https://www.bibsonomy.org/bibtex/20ee2eb582439dba0e179ea92562049c3/kanefendt},
interhash = {8f81a11bc5b1b9b0b095a5501678b655},
intrahash = {0ee2eb582439dba0e179ea92562049c3},
journal = {J.Immunol.Methods},
keywords = {Cell Centrifugation Colloids Count Density Diatrizoate Dioxide Ficoll Gradient Human Humans Leukocyte Lymphocytes Monocytes Polysaccharides Population Separation Silicon blood cells methods pathology},
number = 2,
pages = {203-207},
timestamp = {2010-02-05T11:28:44.000+0100},
title = {Rapid purification of human peripheral blood monocytes by centrifugation through Ficoll-Hypaque and Sepracell-MN},
url = {PM:2837516},
volume = 110,
year = 1988
}