Adenosine receptors as mediators of both cell proliferation and cell
death of cultured human melanoma cells
S. Merighi, P. Mirandola, D. Milani, K. Varani, S. Gessi, K. Klotz, E. Leung, P. Baraldi, and P. Borea. J Invest Dermatol, 119 (4):
923-33(October 2002)Merighi, Stefania Mirandola, Prisco Milani, Daniela Varani, Katia
Gessi, Stefania Klotz, Karl-Norbert Leung, Edward Baraldi, Pier Giovanni
Borea, Pier Andrea United States The Journal of investigative dermatology
J Invest Dermatol. 2002 Oct;119(4):923-33..
Abstract
Adenosine displays contradictory effects on cell growth: it improves
cell proliferation, but it may also induce apoptosis and impair cell
survival. Following the pharmacologic characterization of adenosine
receptor expression on the human melanoma cell line A375, we chose
A375 as our cellular model to define how the extracellular adenosine
signals are conveyed from each receptor. By using selective adenosine
receptor agonists or antagonists, we found that A2A stimulation reduced
cell viability and cell clone formation, whereas, at the same time,
it improved cell proliferation. In support of this finding we demonstrated
that the stimulation of A2A adenosine receptors stably expressed
in Chinese hamster ovary cell clone reproduced deleterious effects
observed in human melanoma cells. A3 stimulation counteracted A2A-induced
cell death but also reduced cell proliferation. Furthermore, we found
that A3 stimulation ensures cell survival. We demonstrated that adenosine
triggers a survival signal via A3 receptor activation and it kills
the cell through A2A receptor inducing a signaling pathway that involves
protein kinase C and mitogen-activated protein kinases.
Merighi, Stefania Mirandola, Prisco Milani, Daniela Varani, Katia
Gessi, Stefania Klotz, Karl-Norbert Leung, Edward Baraldi, Pier Giovanni
Borea, Pier Andrea United States The Journal of investigative dermatology
J Invest Dermatol. 2002 Oct;119(4):923-33.
%0 Journal Article
%1 Merighi2002
%A Merighi, S.
%A Mirandola, P.
%A Milani, D.
%A Varani, K.
%A Gessi, S.
%A Klotz, K. N.
%A Leung, E.
%A Baraldi, P. G.
%A Borea, P. A.
%D 2002
%J J Invest Dermatol
%K 1/metabolism 3 A2A A3 AMP-Dependent Activation Adenosine Adenosine/pharmacology Animals Apoptosis/*physiology C/physiology CHO Cell Cricetinae Cultured Cyclic Division Enzyme Humans Kinase Kinases/metabolism Kinases/physiology Melanoma/*pathology Mitogen-Activated P1/*physiology Protein Purinergic Tumor Receptor
%N 4
%P 923-33
%T Adenosine receptors as mediators of both cell proliferation and cell
death of cultured human melanoma cells
%U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12406340
%V 119
%X Adenosine displays contradictory effects on cell growth: it improves
cell proliferation, but it may also induce apoptosis and impair cell
survival. Following the pharmacologic characterization of adenosine
receptor expression on the human melanoma cell line A375, we chose
A375 as our cellular model to define how the extracellular adenosine
signals are conveyed from each receptor. By using selective adenosine
receptor agonists or antagonists, we found that A2A stimulation reduced
cell viability and cell clone formation, whereas, at the same time,
it improved cell proliferation. In support of this finding we demonstrated
that the stimulation of A2A adenosine receptors stably expressed
in Chinese hamster ovary cell clone reproduced deleterious effects
observed in human melanoma cells. A3 stimulation counteracted A2A-induced
cell death but also reduced cell proliferation. Furthermore, we found
that A3 stimulation ensures cell survival. We demonstrated that adenosine
triggers a survival signal via A3 receptor activation and it kills
the cell through A2A receptor inducing a signaling pathway that involves
protein kinase C and mitogen-activated protein kinases.
@article{Merighi2002,
abstract = {Adenosine displays contradictory effects on cell growth: it improves
cell proliferation, but it may also induce apoptosis and impair cell
survival. Following the pharmacologic characterization of adenosine
receptor expression on the human melanoma cell line A375, we chose
A375 as our cellular model to define how the extracellular adenosine
signals are conveyed from each receptor. By using selective adenosine
receptor agonists or antagonists, we found that A2A stimulation reduced
cell viability and cell clone formation, whereas, at the same time,
it improved cell proliferation. In support of this finding we demonstrated
that the stimulation of A2A adenosine receptors stably expressed
in Chinese hamster ovary cell clone reproduced deleterious effects
observed in human melanoma cells. A3 stimulation counteracted A2A-induced
cell death but also reduced cell proliferation. Furthermore, we found
that A3 stimulation ensures cell survival. We demonstrated that adenosine
triggers a survival signal via A3 receptor activation and it kills
the cell through A2A receptor inducing a signaling pathway that involves
protein kinase C and mitogen-activated protein kinases.},
added-at = {2010-12-14T18:12:02.000+0100},
author = {Merighi, S. and Mirandola, P. and Milani, D. and Varani, K. and Gessi, S. and Klotz, K. N. and Leung, E. and Baraldi, P. G. and Borea, P. A.},
biburl = {https://www.bibsonomy.org/bibtex/218ca666b7fa2c4b540833f1214d8e1fb/pharmawuerz},
endnotereftype = {Journal Article},
interhash = {3d3b6769b23aac3710ed0a60de3ca19e},
intrahash = {18ca666b7fa2c4b540833f1214d8e1fb},
issn = {0022-202X (Print) 0022-202X (Linking)},
journal = {J Invest Dermatol},
keywords = {1/metabolism 3 A2A A3 AMP-Dependent Activation Adenosine Adenosine/pharmacology Animals Apoptosis/*physiology C/physiology CHO Cell Cricetinae Cultured Cyclic Division Enzyme Humans Kinase Kinases/metabolism Kinases/physiology Melanoma/*pathology Mitogen-Activated P1/*physiology Protein Purinergic Tumor Receptor},
month = Oct,
note = {Merighi, Stefania Mirandola, Prisco Milani, Daniela Varani, Katia
Gessi, Stefania Klotz, Karl-Norbert Leung, Edward Baraldi, Pier Giovanni
Borea, Pier Andrea United States The Journal of investigative dermatology
J Invest Dermatol. 2002 Oct;119(4):923-33.},
number = 4,
pages = {923-33},
shorttitle = {Adenosine receptors as mediators of both cell proliferation and cell
death of cultured human melanoma cells},
timestamp = {2010-12-14T18:20:50.000+0100},
title = {Adenosine receptors as mediators of both cell proliferation and cell
death of cultured human melanoma cells},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12406340},
volume = 119,
year = 2002
}