In this Letter we present a new probe, 2-amino-3-hydroxy-2-(hydroxymethyl)propyl 2-(7-hydroxy-2-oxo-2H-chromen-4-yl)acetate (PMR), which can reversibly detect mercuric ions (Hg2+) in HEPES buffer under physiological conditions. Possible interference with other analytes was examined. PMR displays a highly selective decrease of its fluorescence at 460 nm when it reacts with Hg2+. Interestingly, the probe can also be used as a fluorescent turn-on sensor for biologically relevant thiols such as glutathione and cysteine. PMR can be used to determine mercury in living cells.
%0 Journal Article
%1 RN65
%A Garcia-Beltran, O.
%A Mena, N.
%A Berrios, T.A.
%A Castro, E.A.
%A Cassels, B.K.
%A Nunez, M.T.
%A Aliaga, M.E.
%D 2012
%I 2012 Elsevier Ltd.
%J Tetrahedron Letters
%K biothiols, chemodosimeter, coumarin derivatives, design, dqcauchile fluorescent fluorescent, hg(ii) hg2+ hg2+-selectivity, ions, molecules, probe, recognition, rhodamine-based sensor
%N 48
%P 6598-6601
%R 10.1016/j.tetlet.2012.09.111
%T A Selective Fluorescent Probe for the Detection of Mercury (Ii) in Aqueous Media and Its Applications in Living Cells
%U /brokenurl#<Go to ISI>://WOS:000311021600030
%V 53
%X In this Letter we present a new probe, 2-amino-3-hydroxy-2-(hydroxymethyl)propyl 2-(7-hydroxy-2-oxo-2H-chromen-4-yl)acetate (PMR), which can reversibly detect mercuric ions (Hg2+) in HEPES buffer under physiological conditions. Possible interference with other analytes was examined. PMR displays a highly selective decrease of its fluorescence at 460 nm when it reacts with Hg2+. Interestingly, the probe can also be used as a fluorescent turn-on sensor for biologically relevant thiols such as glutathione and cysteine. PMR can be used to determine mercury in living cells.
@article{RN65,
abstract = {In this Letter we present a new probe, 2-amino-3-hydroxy-2-(hydroxymethyl)propyl 2-(7-hydroxy-2-oxo-2H-chromen-4-yl)acetate (PMR), which can reversibly detect mercuric ions (Hg2+) in HEPES buffer under physiological conditions. Possible interference with other analytes was examined. PMR displays a highly selective decrease of its fluorescence at 460 nm when it reacts with Hg2+. Interestingly, the probe can also be used as a fluorescent turn-on sensor for biologically relevant thiols such as glutathione and cysteine. PMR can be used to determine mercury in living cells. },
added-at = {2019-12-04T03:57:35.000+0100},
author = {Garcia-Beltran, O. and Mena, N. and Berrios, T.A. and Castro, E.A. and Cassels, B.K. and Nunez, M.T. and Aliaga, M.E.},
biburl = {https://www.bibsonomy.org/bibtex/2ff4e42d2f3b25dd2ede9e77c77e2cf5c/dqcauchile},
doi = {10.1016/j.tetlet.2012.09.111},
interhash = {ac207aa9623272d3f21605446176a30e},
intrahash = {ff4e42d2f3b25dd2ede9e77c77e2cf5c},
issn = {0040-4039},
journal = {Tetrahedron Letters},
keywords = {biothiols, chemodosimeter, coumarin derivatives, design, dqcauchile fluorescent fluorescent, hg(ii) hg2+ hg2+-selectivity, ions, molecules, probe, recognition, rhodamine-based sensor},
number = 48,
pages = {6598-6601},
publisher = {2012 Elsevier Ltd.},
timestamp = {2019-12-04T03:58:17.000+0100},
title = {A Selective Fluorescent Probe for the Detection of Mercury (Ii) in Aqueous Media and Its Applications in Living Cells},
type = {Journal Article},
url = {/brokenurl#<Go to ISI>://WOS:000311021600030},
volume = 53,
year = 2012
}