%0 Journal Article %1 Banks.1998b %A Banks, R. E. %A Forbes, M. A. %A Kinsey, S. E. %A Stanley, A. %A Ingham, E. %A Walters, C. %A Selby, P. J. %D 1998 %J Br.J Cancer %K A Acid Adult Aged Assay Biological Blood Bone Cells Citrates Collection Edetic Endothelial Enzyme-Linked Factor Factors Female Growth Healing Heparin Humans Immunohistochemistry Immunosorbent Indicators Lymphokines Male Markers Marrow Megakaryocytes Middle Platelets Reagents Reference Research Sensitivity Specificity Specimen Values Vascular Wound and biosynthesis blood cells cytology methods physiology %N 6 %P 956-964 %T Release of the angiogenic cytokine vascular endothelial growth factor (VEGF) from platelets: significance for VEGF measurements and cancer biology %U PM:9528841 %V 77 %X Vascular endothelial growth factor (VEGF) is a potent angiogenic factor with a key role in several pathological processes, including tumour vascularization. Our preliminary observations indicated higher VEGF concentrations in serum samples than in matched plasma samples. We have now demonstrated that this difference is due to the presence of VEGF within platelets and its release upon their activation during coagulation. In eight healthy volunteers, serum VEGF concentrations ranged from 76 to 854 pg ml(-1) and were significantly higher (P < 0.01) than the matched citrated plasma VEGF concentrations, which ranged from < 9 to 42 pg ml(-1). Using platelet-rich plasma, mean (s.d.) platelet VEGF contents of 0.56 (0.36) pg of VEGF 10(-6) platelets were found. Immunocytochemistry demonstrated the cytoplasmic presence of VEGF within megakaryocytes and other cell types within the bone marrow. From examination of the effects of blood sample processing on circulating VEGF concentrations, it is app

@article{Banks.1998b, abstract = {Vascular endothelial growth factor (VEGF) is a potent angiogenic factor with a key role in several pathological processes, including tumour vascularization. Our preliminary observations indicated higher VEGF concentrations in serum samples than in matched plasma samples. We have now demonstrated that this difference is due to the presence of VEGF within platelets and its release upon their activation during coagulation. In eight healthy volunteers, serum VEGF concentrations ranged from 76 to 854 pg ml(-1) and were significantly higher (P < 0.01) than the matched citrated plasma VEGF concentrations, which ranged from < 9 to 42 pg ml(-1). Using platelet-rich plasma, mean (s.d.) platelet VEGF contents of 0.56 (0.36) pg of VEGF 10(-6) platelets were found. Immunocytochemistry demonstrated the cytoplasmic presence of VEGF within megakaryocytes and other cell types within the bone marrow. From examination of the effects of blood sample processing on circulating VEGF concentrations, it is app}, added-at = {2010-02-05T11:28:39.000+0100}, author = {Banks, R. E. and Forbes, M. A. and Kinsey, S. E. and Stanley, A. and Ingham, E. and Walters, C. and Selby, P. J.}, biburl = {https://www.bibsonomy.org/bibtex/2a7d6cab082a8228a14e7c5ba0c50d80c/kanefendt}, interhash = {cac1bcdcf6929e79fbd6e62a04ddd2c8}, intrahash = {a7d6cab082a8228a14e7c5ba0c50d80c}, journal = {Br.J Cancer}, keywords = {A Acid Adult Aged Assay Biological Blood Bone Cells Citrates Collection Edetic Endothelial Enzyme-Linked Factor Factors Female Growth Healing Heparin Humans Immunohistochemistry Immunosorbent Indicators Lymphokines Male Markers Marrow Megakaryocytes Middle Platelets Reagents Reference Research Sensitivity Specificity Specimen Values Vascular Wound and biosynthesis blood cells cytology methods physiology}, number = 6, pages = {956-964}, timestamp = {2010-02-05T11:28:48.000+0100}, title = {Release of the angiogenic cytokine vascular endothelial growth factor (VEGF) from platelets: significance for VEGF measurements and cancer biology}, url = {PM:9528841}, volume = 77, year = 1998 }