Abstract
Fluorescence resonance energy transfer using genetically encoded biosensors
has proven to be a powerful technique to monitor the spatiotemporal
dynamics of cAMP signals stimulated by G(s)-coupled receptors in
living cells. In contrast, real-time imaging of G(i)-mediated cAMP
signals under native conditions remains challenging. Here, we describe
the use of transgenic mice for cAMP imaging in living pituitary slices
and primary pituitary cells. This technique can be widely used to
assess the contribution of various pituitary receptors, including
individual G(i) protein-coupled somatostatin receptors, to the regulation
of cAMP levels under physiologically relevant settings.
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