%0 Journal Article %1 rishmawi_survival_2007 %A Rishmawi, Nabeel %A Ghneim, Raed %A Kattan, Randa %A Ghneim, Riyad %A Zoughbi, Madeleine %A Abu-Diab, Afaf %A Turkuman, Sultan %A Dauodi, Rula %A Shomali, Issa %A Issa, Abed El-Razeq %A Siriani, Issa %A Marzouka, Hiyam %A Schmid, Irmgard %A Hindiyeh, Musa Y %D 2007 %J Journal of Clinical Microbiology %K Bacteriological Colony Count, Factors Feces, Haemophilus Handling, Humans, Microbial Microbial, Neisseria Rectum, Sensitivity Specificity, Specimen Streptococcus Techniques, Temperature, Time Viability, and gonorrhoeae, influenzae, meningitidis, pneumoniae, swab, %N 4 %P 1278--1283 %R 10.1128/JCM.02110-06 %T Survival of fastidious and nonfastidious aerobic bacteria in three bacterial transport swab systems %U http://www.ncbi.nlm.nih.gov/pubmed/17267627 %V 45 %X In the present study, we followed the CLSI procedure M40-A to evaluate three specimen transport systems the new BD CultureSwab MaxV(+), the new Remel BactiSwab, and the Medical Wire & Equipment Transwab for the survival of fastidious and nonfastidious organisms for 0, 6, 24, and 48 h at room temperature. BD CultureSwab MaxV(+) outperformed the other two swabs for the recovery of the three fastidious organisms, Haemophilus influenzae, Neisseria gonorrhoeae, and Neisseria meningitidis for up to 48 h. Indeed, BD CultureSwab MaxV(+) maintained a constant number of viable H. influenzae and N. meningitidis for up to 48 h, and only a 2 log reduction was noted for N. gonorrhoeae, fulfilling the requirements of M40-A guidelines. However, unlike Remel BactiSwab and the Medical Wire & Equipment Transwab, which fulfilled the M40-A requirements for maintaining the viability of Streptococcus pneumoniae, BD CultureSwab MaxV(+) could not maintain the viability of S. pneumoniae reference or clinical strains past 6 h. Excellent overall sensitivity (98\%) (95\% confidence interval, 89.5 to 99.7) was observed when the BD CultureSwab MaxV(+) rectal swabs were compared to the "gold standard" stool cultures. Thus, the BD CultureSwab MaxV(+) rectal swab can be used when investigating gastrointestinal bacterial outbreaks or when health care providers face difficulties in obtaining stool samples, particularly from children.

@article{rishmawi_survival_2007, abstract = {In the present study, we followed the {CLSI} procedure {M40-A} to evaluate three specimen transport systems [the new {BD} {CultureSwab} {MaxV(+),} the new Remel {BactiSwab,} and the Medical Wire \& Equipment Transwab] for the survival of fastidious and nonfastidious organisms for 0, 6, 24, and 48 h at room temperature. {BD} {CultureSwab} {MaxV(+)} outperformed the other two swabs for the recovery of the three fastidious organisms, Haemophilus influenzae, Neisseria gonorrhoeae, and Neisseria meningitidis for up to 48 h. Indeed, {BD} {CultureSwab} {MaxV(+)} maintained a constant number of viable H. influenzae and N. meningitidis for up to 48 h, and only a 2 log reduction was noted for N. gonorrhoeae, fulfilling the requirements of {M40-A} guidelines. However, unlike Remel {BactiSwab} and the Medical Wire \& Equipment Transwab, which fulfilled the {M40-A} requirements for maintaining the viability of Streptococcus pneumoniae, {BD} {CultureSwab} {MaxV(+)} could not maintain the viability of S. pneumoniae reference or clinical strains past 6 h. Excellent overall sensitivity (98\%) (95\% confidence interval, 89.5 to 99.7) was observed when the {BD} {CultureSwab} {MaxV(+)} rectal swabs were compared to the "gold standard" stool cultures. Thus, the {BD} {CultureSwab} {MaxV(+)} rectal swab can be used when investigating gastrointestinal bacterial outbreaks or when health care providers face difficulties in obtaining stool samples, particularly from children.}, added-at = {2011-03-11T10:05:34.000+0100}, author = {Rishmawi, Nabeel and Ghneim, Raed and Kattan, Randa and Ghneim, Riyad and Zoughbi, Madeleine and {Abu-Diab}, Afaf and Turkuman, Sultan and Dauodi, Rula and Shomali, Issa and Issa, Abed {El-Razeq} and Siriani, Issa and Marzouka, Hiyam and Schmid, Irmgard and Hindiyeh, Musa Y}, biburl = {https://www.bibsonomy.org/bibtex/22d8d00d9f4f0c5e6937976e063837b89/jelias}, doi = {10.1128/JCM.02110-06}, interhash = {dd9af424fc819921ade7a0c865552691}, intrahash = {2d8d00d9f4f0c5e6937976e063837b89}, issn = {0095-1137}, journal = {Journal of Clinical Microbiology}, keywords = {Bacteriological Colony Count, Factors Feces, Haemophilus Handling, Humans, Microbial Microbial, Neisseria Rectum, Sensitivity Specificity, Specimen Streptococcus Techniques, Temperature, Time Viability, and gonorrhoeae, influenzae, meningitidis, pneumoniae, swab,}, month = apr, note = {{PMID:} 17267627}, number = 4, pages = {1278--1283}, timestamp = {2011-03-11T10:06:35.000+0100}, title = {Survival of fastidious and nonfastidious aerobic bacteria in three bacterial transport swab systems}, url = {http://www.ncbi.nlm.nih.gov/pubmed/17267627}, volume = 45, year = 2007 }