Simultaneous optical measurements of cytosolic Ca2+ and cAMP in single
cells
M. Harbeck, O. Chepurny, V. Nikolaev, M. Lohse, G. Holz, and M. Roe. Sci STKE, 2006 (353):
pl6(September 2006)Harbeck, Mark C Chepurny, Oleg Nikolaev, Viacheslav O Lohse, Martin
J Holz, George G Roe, Michael W United States Science's STKE : signal
transduction knowledge environment Sci STKE. 2006 Sep 19;2006(353):pl6..
Abstract
Understanding the temporal and spatial integration of the Ca2+ and
adenosine 3',5'-monophosphate (cAMP) signaling pathways requires
concurrent measurements of both second messengers. Here, we describe
an optical technique to simultaneously image cAMP and Ca2+ concentration
gradients in MIN6 mouse insulinoma cells using Epac1-camps, a Forster
(or fluorescence) resonance energy transfer (FRET)-based cAMP biosensor,
and Fura-2, a fluorescent indicator of Ca2+. This real-time imaging
method allows investigation of the dynamic organization and integration
of multiple levels of signal processing in single living cells.
Harbeck, Mark C Chepurny, Oleg Nikolaev, Viacheslav O Lohse, Martin
J Holz, George G Roe, Michael W United States Science's STKE : signal
transduction knowledge environment Sci STKE. 2006 Sep 19;2006(353):pl6.
%0 Journal Article
%1 Harbeck2006
%A Harbeck, M. C.
%A Chepurny, O.
%A Nikolaev, V. O.
%A Lohse, M. J.
%A Holz, G. G.
%A Roe, M. W.
%D 2006
%J Sci STKE
%K *Biosensing AMP/*analysis Animals Calcium/*analysis Cations, Cell Cyclic Cytosol/*chemistry Divalent Dyes Energy Exchange Factors/genetics Fluorescence Fluorescent Fura-2 Guanine Line, Messenger Mice Nucleotide Proteins/biosynthesis Recombinant Reproducibility Resonance Results Second Systems Techniques Transfection Transfer/methods Tumor of
%N 353
%P pl6
%T Simultaneous optical measurements of cytosolic Ca2+ and cAMP in single
cells
%U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16985238
%V 2006
%X Understanding the temporal and spatial integration of the Ca2+ and
adenosine 3',5'-monophosphate (cAMP) signaling pathways requires
concurrent measurements of both second messengers. Here, we describe
an optical technique to simultaneously image cAMP and Ca2+ concentration
gradients in MIN6 mouse insulinoma cells using Epac1-camps, a Forster
(or fluorescence) resonance energy transfer (FRET)-based cAMP biosensor,
and Fura-2, a fluorescent indicator of Ca2+. This real-time imaging
method allows investigation of the dynamic organization and integration
of multiple levels of signal processing in single living cells.
@article{Harbeck2006,
abstract = {Understanding the temporal and spatial integration of the Ca2+ and
adenosine 3',5'-monophosphate (cAMP) signaling pathways requires
concurrent measurements of both second messengers. Here, we describe
an optical technique to simultaneously image cAMP and Ca2+ concentration
gradients in MIN6 mouse insulinoma cells using Epac1-camps, a Forster
(or fluorescence) resonance energy transfer (FRET)-based cAMP biosensor,
and Fura-2, a fluorescent indicator of Ca2+. This real-time imaging
method allows investigation of the dynamic organization and integration
of multiple levels of signal processing in single living cells.},
added-at = {2010-12-14T18:12:02.000+0100},
author = {Harbeck, M. C. and Chepurny, O. and Nikolaev, V. O. and Lohse, M. J. and Holz, G. G. and Roe, M. W.},
biburl = {https://www.bibsonomy.org/bibtex/234d34fcee09c764b4069c57dbe0c40dc/pharmawuerz},
endnotereftype = {Journal Article},
interhash = {3a54ac0b3629dbea20e4aad2c22288fd},
intrahash = {34d34fcee09c764b4069c57dbe0c40dc},
issn = {1525-8882 (Electronic) 1525-8882 (Linking)},
journal = {Sci STKE},
keywords = {*Biosensing AMP/*analysis Animals Calcium/*analysis Cations, Cell Cyclic Cytosol/*chemistry Divalent Dyes Energy Exchange Factors/genetics Fluorescence Fluorescent Fura-2 Guanine Line, Messenger Mice Nucleotide Proteins/biosynthesis Recombinant Reproducibility Resonance Results Second Systems Techniques Transfection Transfer/methods Tumor of},
month = {Sep 19},
note = {Harbeck, Mark C Chepurny, Oleg Nikolaev, Viacheslav O Lohse, Martin
J Holz, George G Roe, Michael W United States Science's STKE : signal
transduction knowledge environment Sci STKE. 2006 Sep 19;2006(353):pl6.},
number = 353,
pages = {pl6},
shorttitle = {Simultaneous optical measurements of cytosolic Ca2+ and cAMP in single
cells},
timestamp = {2010-12-14T18:12:13.000+0100},
title = {Simultaneous optical measurements of cytosolic Ca2+ and cAMP in single
cells},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16985238},
volume = 2006,
year = 2006
}