AIM: To determine the expression and regulation of vascular endothelial growth factor C (VEGF-C), and its receptor VEGFR-3, in human retinal pigment epithelial (RPE) cells and to consider their angiogenic role in choroidal neovascularisation (CNV). METHOD: The expression of VEGF-C and VEGFR-3 in cultured human RPE was confirmed by immunostaining, PCR, western blotting, and ELISA. Cultured RPE cells were exposed to VEGF-A and glucose and VEGF-C and VEGFR-3 changes in gene expression determined by RT-PCR. Secreted VEGF-C protein in conditioned media from RPE was examined by western blotting and ELISA analysis. The ability of VEGF-C to elicit tube formation in choroidal endothelial cells was assayed by an in vitro Matrigel model. RESULT: VEGF-A and glucose upregulated VEGF-C mRNA expression and increased the secretion of VEGF-C protein into the culture medium. VEGF-A, but not glucose alone, stimulated VEGFR-3 mRNA expression. VEGF-C acted synergistically with VEGF-A to promote in vitro tu
%0 Journal Article
%1 Zhao.2006
%A Zhao, B.
%A Ma, A.
%A Cai, J.
%A Boulton, M.
%D 2006
%J Br.J.Ophthalmol.
%K A C Cells Chain Choroidal Cultured Dose-Response Drug Endothelial Epithelial Epithelium Expression Eye Factor Gene Glucose Growth Human Humans Messenger Neovascularization Pigment Polymerase Proteins RNA Reaction Receptor-3 Recombinant Regulation Relationship Research Reverse Synergism Transcriptase Vascular analysis cells chemically cytology drug effects genetics induced metabolism methods of pharmacology protein secretion
%N 8
%P 1052-1059
%T VEGF-A regulates the expression of VEGF-C in human retinal pigment epithelial cells
%U PM:16687456
%V 90
%X AIM: To determine the expression and regulation of vascular endothelial growth factor C (VEGF-C), and its receptor VEGFR-3, in human retinal pigment epithelial (RPE) cells and to consider their angiogenic role in choroidal neovascularisation (CNV). METHOD: The expression of VEGF-C and VEGFR-3 in cultured human RPE was confirmed by immunostaining, PCR, western blotting, and ELISA. Cultured RPE cells were exposed to VEGF-A and glucose and VEGF-C and VEGFR-3 changes in gene expression determined by RT-PCR. Secreted VEGF-C protein in conditioned media from RPE was examined by western blotting and ELISA analysis. The ability of VEGF-C to elicit tube formation in choroidal endothelial cells was assayed by an in vitro Matrigel model. RESULT: VEGF-A and glucose upregulated VEGF-C mRNA expression and increased the secretion of VEGF-C protein into the culture medium. VEGF-A, but not glucose alone, stimulated VEGFR-3 mRNA expression. VEGF-C acted synergistically with VEGF-A to promote in vitro tu
@article{Zhao.2006,
abstract = {AIM: To determine the expression and regulation of vascular endothelial growth factor C (VEGF-C), and its receptor VEGFR-3, in human retinal pigment epithelial (RPE) cells and to consider their angiogenic role in choroidal neovascularisation (CNV). METHOD: The expression of VEGF-C and VEGFR-3 in cultured human RPE was confirmed by immunostaining, PCR, western blotting, and ELISA. Cultured RPE cells were exposed to VEGF-A and glucose and VEGF-C and VEGFR-3 changes in gene expression determined by RT-PCR. Secreted VEGF-C protein in conditioned media from RPE was examined by western blotting and ELISA analysis. The ability of VEGF-C to elicit tube formation in choroidal endothelial cells was assayed by an in vitro Matrigel model. RESULT: VEGF-A and glucose upregulated VEGF-C mRNA expression and increased the secretion of VEGF-C protein into the culture medium. VEGF-A, but not glucose alone, stimulated VEGFR-3 mRNA expression. VEGF-C acted synergistically with VEGF-A to promote in vitro tu},
added-at = {2010-02-05T11:28:39.000+0100},
author = {Zhao, B. and Ma, A. and Cai, J. and Boulton, M.},
biburl = {https://www.bibsonomy.org/bibtex/2a2b0943986aa392c2d193e2d5024f3a4/kanefendt},
interhash = {93150c470b1aa74d33727e6649841503},
intrahash = {a2b0943986aa392c2d193e2d5024f3a4},
journal = {Br.J.Ophthalmol.},
keywords = {A C Cells Chain Choroidal Cultured Dose-Response Drug Endothelial Epithelial Epithelium Expression Eye Factor Gene Glucose Growth Human Humans Messenger Neovascularization Pigment Polymerase Proteins RNA Reaction Receptor-3 Recombinant Regulation Relationship Research Reverse Synergism Transcriptase Vascular analysis cells chemically cytology drug effects genetics induced metabolism methods of pharmacology protein secretion},
number = 8,
pages = {1052-1059},
timestamp = {2010-02-05T11:28:42.000+0100},
title = {VEGF-A regulates the expression of VEGF-C in human retinal pigment epithelial cells},
url = {PM:16687456},
volume = 90,
year = 2006
}