%0 Journal Article %1 Nikolaev2006c %A Nikolaev, V. O. %A Lohse, M. J. %D 2006 %J Physiology (Bethesda) %K AMP-Dependent AMP/analysis/*metabolism Animals Antiporters/physiology Biosensing Calcium/physiology Cation Cells/chemistry/*metabolism Channels Channels/physiology Cyclic Cytosol/chemistry Electrophysiology/*methods Exchange Factors/analysis/physiology Fluorescence/*methods Guanine Humans Hydrolysis Ion Kinases/physiology Microscopy, Nucleotide Nucleotide-Gated Protein Radioimmunoassay Techniques %P 86-92 %T Monitoring of cAMP synthesis and degradation in living cells %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16565474 %V 21 %X cAMP is an important second messenger with a plethora of cellular effects and biological roles. To monitor and visualize cAMP in intact living cells, electrophysiological and fluorescent methods have been developed based on activation of all three types of cAMP effectors: protein kinase A, cyclic nucleotide-gated channels, and exchange protein directly activated by cAMP. In this review, we describe and compare these techniques in terms of their robustness, sensitivity and spatio-temporal resolution.

@article{Nikolaev2006c, abstract = {cAMP is an important second messenger with a plethora of cellular effects and biological roles. To monitor and visualize cAMP in intact living cells, electrophysiological and fluorescent methods have been developed based on activation of all three types of cAMP effectors: protein kinase A, cyclic nucleotide-gated channels, and exchange protein directly activated by cAMP. In this review, we describe and compare these techniques in terms of their robustness, sensitivity and spatio-temporal resolution.}, added-at = {2010-12-14T18:12:02.000+0100}, author = {Nikolaev, V. O. and Lohse, M. J.}, biburl = {https://www.bibsonomy.org/bibtex/295826cf983fe7ba996de4f12458d4e02/pharmawuerz}, endnotereftype = {Journal Article}, interhash = {58424c782b936c3de31000716ddaba2d}, intrahash = {95826cf983fe7ba996de4f12458d4e02}, issn = {1548-9213 (Print) 1548-9213 (Linking)}, journal = {Physiology (Bethesda)}, keywords = {AMP-Dependent AMP/analysis/*metabolism Animals Antiporters/physiology Biosensing Calcium/physiology Cation Cells/chemistry/*metabolism Channels Channels/physiology Cyclic Cytosol/chemistry Electrophysiology/*methods Exchange Factors/analysis/physiology Fluorescence/*methods Guanine Humans Hydrolysis Ion Kinases/physiology Microscopy, Nucleotide Nucleotide-Gated Protein Radioimmunoassay Techniques}, month = Apr, note = {Nikolaev, Viacheslav O Lohse, Martin J Review United States Physiology (Bethesda, Md.) Physiology (Bethesda). 2006 Apr;21:86-92.}, pages = {86-92}, shorttitle = {Monitoring of cAMP synthesis and degradation in living cells}, timestamp = {2010-12-14T18:12:27.000+0100}, title = {Monitoring of cAMP synthesis and degradation in living cells}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16565474}, volume = 21, year = 2006 }