Vascular endothelial growth factor receptor-3 (VEGFR-3) and its ligands, vascular endothelial growth factor-C (VEGF-C) and -D (VEGF-D), are the major molecules involved in developmental and pathological lymphangiogenesis. Here we describe for the first time the development of a specific indirect enzyme-linked immunosorbent assay (ELISA) for the quantification of VEGFR-3 in different human cell and tissue lysates. A combination of the goat polyclonal anti-VEGFR-3 antibody and the mouse monoclonal anti-human VEGFR-3 antibody was used. The assay was highly sensitive and reproducible with a detection range of 0.2-25 ng/ml. The assay was specific for VEGFR-3, with no cross-reactivity to VEGFR-1 or VEGFR-2. Complex formation with VEGF-C and VEGF-D had no effect on the sensitivity of the assay. The VEGFR-3 concentration in the lysates of cultured human dermal microvascular endothelial cells was 14-fold higher than in the lysates from human umbilical vein endothelial cells. In human kidney, br
%0 Journal Article
%1 Bando.2004
%A Bando, H.
%A Brokelmann, M.
%A Toi, M.
%A Alitalo, K.
%A Sleeman, J. P.
%A Sipos, B.
%A Grone, H. J.
%A Weich, H. A.
%D 2004
%J Int.J.Cancer
%K Animals Antibodies Assay Carcinoma Cells Chemistry Cultured Distribution Endothelial Enzyme-Linked Factor Goats Growth Human Humans Immunosorbent Kidney Ligands Lung Mice Monoclonal Neoplasms Neovascularization Pathologic RANGE Receptor-3 Research Sensitivity Specificity Tissue Tumor Vascular analysis and cells diagnostic immunology methods pathology protein use
%N 2
%P 184-191
%T Immunodetection and quantification of vascular endothelial growth factor receptor-3 in human malignant tumor tissues
%U PM:15197769
%V 111
%X Vascular endothelial growth factor receptor-3 (VEGFR-3) and its ligands, vascular endothelial growth factor-C (VEGF-C) and -D (VEGF-D), are the major molecules involved in developmental and pathological lymphangiogenesis. Here we describe for the first time the development of a specific indirect enzyme-linked immunosorbent assay (ELISA) for the quantification of VEGFR-3 in different human cell and tissue lysates. A combination of the goat polyclonal anti-VEGFR-3 antibody and the mouse monoclonal anti-human VEGFR-3 antibody was used. The assay was highly sensitive and reproducible with a detection range of 0.2-25 ng/ml. The assay was specific for VEGFR-3, with no cross-reactivity to VEGFR-1 or VEGFR-2. Complex formation with VEGF-C and VEGF-D had no effect on the sensitivity of the assay. The VEGFR-3 concentration in the lysates of cultured human dermal microvascular endothelial cells was 14-fold higher than in the lysates from human umbilical vein endothelial cells. In human kidney, br
@article{Bando.2004,
abstract = {Vascular endothelial growth factor receptor-3 (VEGFR-3) and its ligands, vascular endothelial growth factor-C (VEGF-C) and -D (VEGF-D), are the major molecules involved in developmental and pathological lymphangiogenesis. Here we describe for the first time the development of a specific indirect enzyme-linked immunosorbent assay (ELISA) for the quantification of VEGFR-3 in different human cell and tissue lysates. A combination of the goat polyclonal anti-VEGFR-3 antibody and the mouse monoclonal anti-human VEGFR-3 antibody was used. The assay was highly sensitive and reproducible with a detection range of 0.2-25 ng/ml. The assay was specific for VEGFR-3, with no cross-reactivity to VEGFR-1 or VEGFR-2. Complex formation with VEGF-C and VEGF-D had no effect on the sensitivity of the assay. The VEGFR-3 concentration in the lysates of cultured human dermal microvascular endothelial cells was 14-fold higher than in the lysates from human umbilical vein endothelial cells. In human kidney, br},
added-at = {2010-02-05T11:28:39.000+0100},
author = {Bando, H. and Brokelmann, M. and Toi, M. and Alitalo, K. and Sleeman, J. P. and Sipos, B. and Grone, H. J. and Weich, H. A.},
biburl = {https://www.bibsonomy.org/bibtex/2351bf2b8227b115843af8eccc2fa1316/kanefendt},
interhash = {ac90174bf3dbb2a0c8cd16c5ccb9694b},
intrahash = {351bf2b8227b115843af8eccc2fa1316},
journal = {Int.J.Cancer},
keywords = {Animals Antibodies Assay Carcinoma Cells Chemistry Cultured Distribution Endothelial Enzyme-Linked Factor Goats Growth Human Humans Immunosorbent Kidney Ligands Lung Mice Monoclonal Neoplasms Neovascularization Pathologic RANGE Receptor-3 Research Sensitivity Specificity Tissue Tumor Vascular analysis and cells diagnostic immunology methods pathology protein use},
number = 2,
pages = {184-191},
timestamp = {2010-02-05T11:28:43.000+0100},
title = {Immunodetection and quantification of vascular endothelial growth factor receptor-3 in human malignant tumor tissues},
url = {PM:15197769},
volume = 111,
year = 2004
}